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Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene.


ABSTRACT: The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC.

SUBMITTER: Lee H 

PROVIDER: S-EPMC4742916 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene.

Lee Hyunah H   Nam Donggyu D   Choi Jae-Kyung JK   Araúzo-Bravo Marcos J MJ   Kwon Soon-Yong SY   Zaehres Holm H   Lee Taehee T   Park Chan Young CY   Kang Hyun-Wook HW   Schöler Hans R HR   Kim Jeong Beom JB  

Scientific reports 20160205


The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which comple  ...[more]

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