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In Vivo, In Vitro, and In Silico Characterization of Peptoids as Antimicrobial Agents.


ABSTRACT: Bacterial resistance to conventional antibiotics is a global threat that has spurred the development of antimicrobial peptides (AMPs) and their mimetics as novel anti-infective agents. While the bioavailability of AMPs is often reduced due to protease activity, the non-natural structure of AMP mimetics renders them robust to proteolytic degradation, thus offering a distinct advantage for their clinical application. We explore the therapeutic potential of N-substituted glycines, or peptoids, as AMP mimics using a multi-faceted approach that includes in silico, in vitro, and in vivo techniques. We report a new QSAR model that we developed based on 27 diverse peptoid sequences, which accurately correlates antimicrobial peptoid structure with antimicrobial activity. We have identified a number of peptoids that have potent, broad-spectrum in vitro activity against multi-drug resistant bacterial strains. Lastly, using a murine model of invasive S. aureus infection, we demonstrate that one of the best candidate peptoids at 4 mg/kg significantly reduces with a two-log order the bacterial counts compared with saline-treated controls. Taken together, our results demonstrate the promising therapeutic potential of peptoids as antimicrobial agents.

SUBMITTER: Czyzewski AM 

PROVIDER: S-EPMC4744035 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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In Vivo, In Vitro, and In Silico Characterization of Peptoids as Antimicrobial Agents.

Czyzewski Ann M AM   Jenssen Håvard H   Fjell Christopher D CD   Waldbrook Matt M   Chongsiriwatana Nathaniel P NP   Yuen Eddie E   Hancock Robert E W RE   Barron Annelise E AE  

PloS one 20160205 2


Bacterial resistance to conventional antibiotics is a global threat that has spurred the development of antimicrobial peptides (AMPs) and their mimetics as novel anti-infective agents. While the bioavailability of AMPs is often reduced due to protease activity, the non-natural structure of AMP mimetics renders them robust to proteolytic degradation, thus offering a distinct advantage for their clinical application. We explore the therapeutic potential of N-substituted glycines, or peptoids, as A  ...[more]

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