Ontology highlight
ABSTRACT:
SUBMITTER: Roche J
PROVIDER: S-EPMC4744140 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Roche Julien J Ying Jinfa J Bax Ad A
Journal of biomolecular NMR 20151210 1
Provided that care is taken in adjusting the WATERGATE element of a (1)H-(15)N TROSY-HSQC experiment, such that neither the water magnetization nor the (1)H(α) protons are inverted by its final 180° pulse, (3)JHNHα couplings can be measured directly from splittings in the (1)H dimension of the spectrum. With band-selective (1)H decoupling, very high (15)N resolution can be achieved. A complete set of (3)JHNHα values, ranging from 3.4 to 10.1 Hz was measured for the 56-residue third domain of IgG ...[more]