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A molecular nanodevice for targeted degradation of mRNA during protein synthesis.

ABSTRACT: RNase H is an endonuclease that catalyzes the cleavage of RNA. Because it only acts on RNA in RNA:DNA hybrids, RNase H can be used for targeted degradation of RNA when used in combination with antisense oligodeoxyribonucleotides (ASODNs) designed against a specific sequence of the target RNA. In this study, ASODN and RNase H were co-conjugated on magnetic nanoparticles. The resulting nanoparticles, having integrated functions of probing and processing target RNA, were able to remove target mRNA sequences more effectively than free ASODNs. The paramagnetic property of the nanoparticles also enabled timed engagement and disengagement of the RNA-degrading components in a given system, and these nanoparticles were able to be used for ON/OFF control of gene expression during cell-free protein synthesis reactions.

SUBMITTER: Lee KH

PROVIDER: S-EPMC4746582 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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A molecular nanodevice for targeted degradation of mRNA during protein synthesis.

Lee Kyung-Ho KH Min Seung-Eui SE Kim Haseong H Lee Seung-Goo SG Kim Dong-Myung DM

Scientific reports 20160209

RNase H is an endonuclease that catalyzes the cleavage of RNA. Because it only acts on RNA in RNA:DNA hybrids, RNase H can be used for targeted degradation of RNA when used in combination with antisense oligodeoxyribonucleotides (ASODNs) designed against a specific sequence of the target RNA. In this study, ASODN and RNase H were co-conjugated on magnetic nanoparticles. The resulting nanoparticles, having integrated functions of probing and processing target RNA, were able to remove target mRNA ...[more]

PMID: 26857021

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