Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC4747823 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Li Jiannong J Fang Bin B Kinose Fumi F Bai Yun Y Kim Jae-Young JY Chen Yian A YA Rix Uwe U Koomen John M JM Haura Eric B EB
Molecular cancer therapeutics 20160115 2
To overcome hurdles in identifying key kinases in small cell lung cancer (SCLC), we integrated a target-agnostic phenotypic screen of kinase inhibitors with target identification using activity-based protein profiling (ABPP) in which a desthiobiotin-ATP probe was used. We screened 21 SCLC cell lines with known c-MYC amplification status for alterations in viability using a chemical library of 235 small-molecule kinase inhibitors. One screen hit compound was interrogated with ABPP, and, through t ...[more]