S-EPMC4749848 - Polymorphisms in NOS3, MTHFR, APOB and TNF-? Genes and Risk of Coronary Atherosclerotic Lesions in Iranian Patients. - OmicsDI

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Polymorphisms in NOS3, MTHFR, APOB and TNF-? Genes and Risk of Coronary Atherosclerotic Lesions in Iranian Patients.

ABSTRACT: BACKGROUND:Atherosclerosis is a complex multifocal arterial disease involving interactions between multiple genetic and environmental factors. OBJECTIVES:In the present study, we investigated the possible association between NOS3 (rs1799983), MTHFR (rs1801133), APOB (rs5742904) and TNF-? (rs361525) polymorphisms and the risk of coronary atherosclerotic lesions in Iranian patients. PATIENTS AND METHODS:In the case-control study, 108 patients with coronary atherosclerosis disease and 95 control subjects with no family history of cardiovascular disease were enrolled. Genotypes for NOS3, MTHFR, APOB and TNF-? polymorphisms were identified using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). RESULTS:We specifically detected the NOS3 TT genotype in 12 patients (11.11%) and did not find the same genotype in any of the controls. The frequencies of T allele in patients and the controls were 24% and 17.8%, respectively. The prevalence of the MTHFR TT genotype was 16.7% in patients and 2.2% in control groups. The prevalence of the APOB-100 (R3500Q) mutation in this patient population was 0%. The frequency of the A allele in the TNF-? gene was 11.1% and 11% in patients and controls, respectively, and the AA genotype was undetected. CONCLUSIONS:Our results show a significant association of NOS3 and MTHFR gene polymorphisms with coronary atherosclerotic lesions. Therefore, these variants might influence the risk of coronary artery disease, specifically in the Iranian population.

SUBMITTER: Heidari MM

PROVIDER: S-EPMC4749848 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Polymorphisms in NOS3, MTHFR, APOB and TNF-α Genes and Risk of Coronary Atherosclerotic Lesions in Iranian Patients.

Heidari Mohammad Mehdi MM Khatami Mehri M Hadadzadeh Mehdi M Kazemi Mahbobeh M Mahamed Sahar S Malekzadeh Pegah P Mirjalili Massomeh M

Research in cardiovascular medicine 20151223 1

<h4>Background</h4>Atherosclerosis is a complex multifocal arterial disease involving interactions between multiple genetic and environmental factors.<h4>Objectives</h4>In the present study, we investigated the possible association between NOS3 (rs1799983), MTHFR (rs1801133), APOB (rs5742904) and TNF-α (rs361525) polymorphisms and the risk of coronary atherosclerotic lesions in Iranian patients.<h4>Patients and methods</h4>In the case-control study, 108 patients with coronary atherosclerosis dis ...[more]

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