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Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification.

ABSTRACT: Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.

SUBMITTER: Koide R

PROVIDER: S-EPMC4751125 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification.

Koide Rie R Sakaguchi Shoichi S Ogawa Makoto M Miyazawa Takayuki T

The Journal of veterinary medical science 20150811 1

Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. W ...[more]

PMID: 26268665

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