Development of Antibiotic Resistance during Simulated Treatment of Pseudomonas aeruginosa in Chemostats.

ABSTRACT: During treatment of infections with antibiotics in critically ill patients in the intensive care resistance often develops. This study aims to establish whether under those conditions this resistance can develop de novo or that genetic exchange between bacteria is by necessity involved. Chemostat cultures of Pseudomonas aeruginosa were exposed to treatment regimes with ceftazidime and meropenem that simulated conditions expected in patient plasma. Development of antibiotic resistance was monitored and mutations in resistance genes were searched for by sequencing PCR products. Even at the highest concentrations that can be expected in patients, sufficient bacteria survived in clumps of filamentous cells to recover and grow out after 3 to 5 days. At the end of a 7 days simulated treatment, t

SUBMITTER: Feng Y 

PROVIDER: S-EPMC4752458 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature