An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer.

ABSTRACT: The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by direct lysis from athalassohaline soil, it was observed that, upon addition of poly ethylene glycol (PEG), isopropanol or absolute ethanol for precipitation of DNA, salt precipitates out and affecting DNA yield significantly. Therefore, indirect lysis method was optimized for extraction of environmental DNA from such soil containing high salts and low microbial biomass (CFU 4.3 × 10⁴ per gram

SUBMITTER: Narayan A 

PROVIDER: S-EPMC4752949 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature