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Effective Soil Extraction Method for Cultivating Previously Uncultured Soil Bacteria.


ABSTRACT: Here, a new medium, named intensive soil extract medium (ISEM), based on new soil extract (NSE) using 80% methanol, was used to efficiently isolate previously uncultured bacteria and new taxonomic candidates, which accounted for 49% and 55% of the total isolates examined (n = 258), respectively. The new isolates were affiliated with seven phyla (Proteobacteria, Acidobacteria, Firmicutes, Actinobacteria, Verrucomicrobia, Planctomycetes, and Bacteroidetes). The result of chemical analysis showed that NSE included more diverse components of low-molecular-weight organic substances than two conventional soil extracts made using distilled water. Cultivation of previously uncultured bacteria is expected to extend knowledge through the discovery of new phenotypic, physiological, and functional properties and even roles of unknown genes.IMPORTANCE Both metagenomics and single-cell sequencing can detect unknown genes from uncultured microbial strains in environments, and either method may find the significant potential metabolites and roles of these strains. However, such gene/genome-based techniques do not allow detailed investigations that are possible with cultures. To solve this problem, various approaches for cultivation of uncultured bacteria have been developed, but there are still difficulties in maintaining pure cultures by subculture.

SUBMITTER: Nguyen TM 

PROVIDER: S-EPMC6275337 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Effective Soil Extraction Method for Cultivating Previously Uncultured Soil Bacteria.

Nguyen Tuan Manh TM   Seo Chan C   Ji Moongi M   Paik Man-Jeong MJ   Myung Seung-Woon SW   Kim Jaisoo J  

Applied and environmental microbiology 20181130 24


Here, a new medium, named intensive soil extract medium (ISEM), based on new soil extract (NSE) using 80% methanol, was used to efficiently isolate previously uncultured bacteria and new taxonomic candidates, which accounted for 49% and 55% of the total isolates examined (<i>n</i> = 258), respectively. The new isolates were affiliated with seven phyla (<i>Proteobacteria</i>, <i>Acidobacteria</i>, <i>Firmicutes</i>, <i>Actinobacteria</i>, <i>Verrucomicrobia</i>, <i>Planctomycetes</i>, and <i>Bact  ...[more]

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