Low Interferon Relative-Response to Cytomegalovirus Is Associated with Low Likelihood of Intrauterine Transmission of the Virus.

ABSTRACT:

Background

Congenital Cytomegalovirus (CMV) is a very common intrauterine infection which can cause severe mental and hearing impairments. Notably, only 40% of primarily infected women transmit CMV to the fetus. CMV-specific T-cell response has a role in CMV disease but individual immune heterogeneity precludes reliable correlation between measurable T-cells response and intrauterine transmission.

Study aim

To establish a correlation between maternal T-cells response and fetal CMV transmission using an individual normalized immune response.

Methods

We analyzed IFN-? secretion upon whole blood stimulation from primary CMV-infected pregnant women, with either CMV-peptides or PHA-mitogen.

Results

We established a new normalization method of individual IFN-? response to CMV by defining the ratio between specific-CMV response and non-specific mitogen response (defined as IFN-? relative response, RR), aiming to overcome high person-to-person immune variability. We found a unique subpopulation of women with low IFN-? RR strongly correlated with absence of transmission. IFN-? RR lower than 1.8% (threshold determined by ROC analysis) reduces the pre-test probability of transmission from 40% to 8%, revealing an unexpected link between low IFN-? RR and non-transmission.

Conclusion

In pregnant women with primary CMV infection, low IFN-? RR is associated with low risk of transmission.

SUBMITTER: Eldar-Yedidia Y 

PROVIDER: S-EPMC4755570 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature