DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population.

ABSTRACT:

Background

Durum wheat (Triticum turgidum L.) is a cereal crop widely grown in the Mediterranean regions; the amber grain is mainly used for the production of pasta, couscous and typical breads. Single nucleotide polymorphism (SNP) detection technologies and high-throughput mutation induction represent a new challenge in wheat breeding to identify allelic variation in large populations. The TILLING strategy makes use of traditional chemical mutagenesis followed by screening for single base mismatches to identify novel mutant loci. Although TILLING has been combined to several sensitive pre-screening methods for SNP analysis, most rely on expensive equipment. Recently, a new low cost and time saving DHPLC protocol has been used in molecular human diagnostic to detect unknown mutatio

SUBMITTER: Colasuonno P 

PROVIDER: S-EPMC4756519 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature