Blue Light Modulates Murine Microglial Gene Expression in the Absence of Optogenetic Protein Expression.

ABSTRACT: Neural optogenetic applications over the past decade have steadily increased; however the effects of commonly used blue light paradigms on surrounding, non-optogenetic protein-expressing CNS cells are rarely considered, despite their simultaneous exposure. Here we report that blue light (450 nm) repetitively delivered in both long-duration boluses and rapid optogenetic bursts gene-specifically altered basal expression of inflammatory and neurotrophic genes in immortalized and primary murine wild type microglial cultures. In addition, blue light reduced pro-inflammatory gene expression in microglia activated with lipopolysaccharide. These results demonstrate previously unreported, off-target effects of blue light in cells not expressing optogenetic constructs. The unexpected gene modulatory

SUBMITTER: Cheng KP 

PROVIDER: S-EPMC4756664 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature