Dataset Information

Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis.

ABSTRACT: A novel bacterium capable of utilizing 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) as the sole carbon and energy source was isolated from a contaminated soil which was identified as Stenotrophomonas sp. DDT-1 based on morphological characteristics, BIOLOG GN2 microplate profile, and 16S rDNA phylogeny. Genome sequencing and functional annotation of the isolate DDT-1 showed a 4,514,569 bp genome size, 66.92% GC content, 4,033 protein-coding genes, and 76 RNA genes including 8 rRNA genes. Totally, 2,807 protein-coding genes were assigned to Clusters of Orthologous Groups (COGs), and 1,601 protein-coding genes were mapped to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. The degradation half-lives of DDT increased with substrate concentration from 0.1 to 10.0 mg/l, whereas decreased with temperature from 15?°C to 35?°C. Neutral condition was the most favorable for DDT biodegradation. Based on genome annotation of DDT degradation genes and the metabolites detected by GC-MS, a mineralization pathway was proposed for DDT biodegradation in which it was orderly converted into DDE/DDD, DDMU, DDOH, and DDA via dechlorination, hydroxylation, and carboxylation, and ultimately mineralized to carbon dioxide. The results indicate that the isolate DDT-1 is a promising bacterial resource for the removal or detoxification of DDT residues in the environment.

SUBMITTER: Pan X

PROVIDER: S-EPMC4758049 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis.

Pan Xiong X Lin Dunli D Zheng Yuan Y Zhang Qian Q Yin Yuanming Y Cai Lin L Fang Hua H Yu Yunlong Y

Scientific reports 20160218

A novel bacterium capable of utilizing 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) as the sole carbon and energy source was isolated from a contaminated soil which was identified as Stenotrophomonas sp. DDT-1 based on morphological characteristics, BIOLOG GN2 microplate profile, and 16S rDNA phylogeny. Genome sequencing and functional annotation of the isolate DDT-1 showed a 4,514,569 bp genome size, 66.92% GC content, 4,033 protein-coding genes, and 76 RNA genes including 8 rRNA genes. ...[more]

PMID: 26888254

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Dataset Information

Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis.

Publications

Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis.

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