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Ultra-sensitive immunoassay biosensors using hybrid plasmonic-biosilica nanostructured materials.

ABSTRACT: We experimentally demonstrate an ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles. As the nature-created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface-enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic-biosilica nanostructured materials that are functionalized with goat anti-mouse IgG. Our experimental results show that diatom frustules improve the detection limit of mouse IgG to 10 pg/mL, which is ˜100× better than conventional colloidal SERS sensors on flat glass. Ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles.

SUBMITTER: Yang J

PROVIDER: S-EPMC4758816 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Ultra-sensitive immunoassay biosensors using hybrid plasmonic-biosilica nanostructured materials.

Yang Jing J Zhen Le L Ren Fanghui F Campbell Jeremy J Rorrer Gregory L GL Wang Alan X AX

Journal of biophotonics 20140925 8

We experimentally demonstrate an ultra-sensitive immunoassay biosensor using diatom biosilica with self-assembled plasmonic nanoparticles. As the nature-created photonic crystal structures, diatoms have been adopted to enhance surface plasmon resonances of metal nanoparticles on the surfaces of diatom frustules and to increase the sensitivity of surface-enhanced Raman scattering (SERS). In this study, a sandwich SERS immunoassay is developed based on the hybrid plasmonic-biosilica nanostructured ...[more]

PMID: 25256544

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