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Differential changes in gene expression in human neutrophils following TNF-? stimulation: Up-regulation of anti-apoptotic proteins and down-regulation of proteins involved in death receptor signaling.

ABSTRACT: Responses of human neutrophils to TNF-? are complex and multifactorial. Exposure of human neutrophils to TNF-? in vitro primes the respiratory burst, delays apoptosis and induces the expression of several genes including chemokines, and TNF-? itself. This study aimed to determine the impact of TNF-? exposure on the expression of neutrophil genes and proteins that regulate apoptosis. Quantitative PCR and RNA-Seq, identified changes in expression of several apoptosis regulating genes in response to TNF-? exposure. Up-regulated genes included TNF-? itself, and several anti-apoptotic genes, including BCL2A1, CFLAR (cFLIP) and TNFAIP3, whose mRNA levels increased above control values by between 4-20 fold (n?=?3, P?

SUBMITTER: Chiewchengchol D 

PROVIDER: S-EPMC4768069 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Differential changes in gene expression in human neutrophils following TNF-α stimulation: Up-regulation of anti-apoptotic proteins and down-regulation of proteins involved in death receptor signaling.

Chiewchengchol Direkrit D   Wright Helen L HL   Thomas Huw B HB   Lam Connie W CW   Roberts Kate J KJ   Hirankarn Nattiya N   Beresford Michael W MW   Moots Robert J RJ   Edwards Steven W SW  

Immunity, inflammation and disease 20151202 1

Responses of human neutrophils to TNF-α are complex and multifactorial. Exposure of human neutrophils to TNF-α in vitro primes the respiratory burst, delays apoptosis and induces the expression of several genes including chemokines, and TNF-α itself. This study aimed to determine the impact of TNF-α exposure on the expression of neutrophil genes and proteins that regulate apoptosis. Quantitative PCR and RNA-Seq, identified changes in expression of several apoptosis regulating genes in response t  ...[more]

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