Ontology highlight
ABSTRACT:
SUBMITTER: Rajendram M
PROVIDER: S-EPMC4771187 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Rajendram Manohary M Zhang Leili L Reynolds Bradley J BJ Auer George K GK Tuson Hannah H HH Ngo Khanh V KV Cox Michael M MM Yethiraj Arun A Cui Qiang Q Weibel Douglas B DB
Molecular cell 20151017 3
We characterize the interaction of RecA with membranes in vivo and in vitro and demonstrate that RecA binds tightly to the anionic phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Using computational models, we identify two regions of RecA that interact with PG and CL: (1) the N-terminal helix and (2) loop L2. Mutating these regions decreased the affinity of RecA to PG and CL in vitro. Using 3D super-resolution microscopy, we demonstrate that depleting Escherichia coli PG and CL alt ...[more]