Development of a recombinant human ovarian (BG1) cell line containing estrogen receptor ? and ? for improved detection of estrogenic/antiestrogenic chemicals.

ABSTRACT: Estrogenic endocrine-disrupting chemicals are found in environmental and biological samples, commercial and consumer products, food, and numerous other sources. Given their ubiquitous nature and potential for adverse effects, a critical need exists for rapidly detecting these chemicals. The authors developed an estrogen-responsive recombinant human ovarian (BG1Luc4E2) cell line recently accepted by the US Environmental Protection Agency (USEPA) and Organisation for Economic Co-operation and Development (OECD) as a bioanalytical method to detect estrogen receptor (ER) agonists/antagonists. Unfortunately, these cells appear to contain only 1 of the 2 known ER isoforms, ER? but not ER?, and the differential ligand selectivity of these ERs indicates that the currently accepted screening method only detects a subset of total estrogenic chemicals. To improve the estrogen screening bioassay, BG1Luc4E2 cells were stably transfected with an ER? expression plasmid and positive clones identified using ER?-selective ligands (genistein and Br-ER?-041). A highly responsive clone (BG1LucER?c9) was identified that exhibited greater sensitivity and responsiveness to ER?-selective ligands than BG1Luc4E2 cells, and quantitative reverse-transcription polymerase chain reaction confirmed the presence of ER? expression in these cells. Screening of pesticides and industrial chemicals identified chemicals that preferentially stimulated ER?-dependent reporter gene expression. Together, these results not only demonstrate the utility of this dual-ER recombinant cell line for detecting a broader range of estrogenic chemicals than the current BG1Luc4E2 cell line, but screening with both cell lines allows identification of ER?- and ER?-selective chemicals.

SUBMITTER: Brennan JC 

PROVIDER: S-EPMC4772679 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature