Ontology highlight
ABSTRACT:
SUBMITTER: Eckerdt F
PROVIDER: S-EPMC4772731 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Eckerdt Frank F Alvarez Angel A Bell Jonathan J Arvanitis Constadina C Iqbal Asneha A Arslan Ahmet D AD Hu Bo B Cheng Shi-Yuan SY Goldman Stewart S Platanias Leonidas C LC
BioTechniques 20160101 1
Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, ...[more]