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A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids.

ABSTRACT: Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

SUBMITTER: Eckerdt F

PROVIDER: S-EPMC4772731 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids.

Eckerdt Frank F Alvarez Angel A Bell Jonathan J Arvanitis Constadina C Iqbal Asneha A Arslan Ahmet D AD Hu Bo B Cheng Shi-Yuan SY Goldman Stewart S Platanias Leonidas C LC

BioTechniques 20160101 1

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, ...[more]

PMID: 26757811

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