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Two-photon absorption properties of fluorescent proteins.


ABSTRACT: Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications.

SUBMITTER: Drobizhev M 

PROVIDER: S-EPMC4772972 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Two-photon absorption properties of fluorescent proteins.

Drobizhev Mikhail M   Makarov Nikolay S NS   Tillo Shane E SE   Hughes Thomas E TE   Rebane Aleksander A  

Nature methods 20110428 5


Two-photon excitation of fluorescent proteins is an attractive approach for imaging living systems. Today researchers are eager to know which proteins are the brightest and what the best excitation wavelengths are. Here we review the two-photon absorption properties of a wide variety of fluorescent proteins, including new far-red variants, to produce a comprehensive guide to choosing the right fluorescent protein and excitation wavelength for two-photon applications. ...[more]

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