Ontology highlight
ABSTRACT:
SUBMITTER: Molina RS
PROVIDER: S-EPMC7747914 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Molina Rosana S RS King Jonathan J Franklin Jacob J Clack Nathan N McRaven Christopher C Goncharov Vasily V Flickinger Daniel D Svoboda Karel K Drobizhev Mikhail M Hughes Thomas E TE
Biomedical optics express 20201117 12
Two-photon microscopy together with fluorescent proteins and fluorescent protein-based biosensors are commonly used tools in neuroscience. To enhance their experimental scope, it is important to optimize fluorescent proteins for two-photon excitation. Directed evolution of fluorescent proteins under one-photon excitation is common, but many one-photon properties do not correlate with two-photon properties. A simple system for expressing fluorescent protein mutants is <i>E. coli</i> colonies on a ...[more]