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A bead-based cleavage method for large-scale identification of protease substrates.


ABSTRACT: Proteolysis is a major form of post translational modification which occurs when a protease cleaves peptide bonds in a target protein to modify its activity. Tracking protease substrates is indispensable for understanding its cellular functions. However, it is difficult to directly identify protease substrates because the end products of proteolysis, the cleaved protein fragments, must be identified among the pool of cellular proteins. Here we present a bead-based cleavage approach using immobilized proteome as the screening library to identify protease substrates. This method enables efficient separation of proteolyzed proteins from background protein mixture. Using caspase-3 as the model protease, we have identified 1159 high confident substrates, among which, strikingly, 43.9% of substrates undergo degradation during apoptosis. The huge number of substrates and positive support of in vivo evidence indicate that the BBC method is a powerful tool for protease substrates identification.

SUBMITTER: Wang C 

PROVIDER: S-EPMC4776233 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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A bead-based cleavage method for large-scale identification of protease substrates.

Wang Chunli C   Ye Mingliang M   Wei Xiaoluan X   Bian Yangyang Y   Cheng Kai K   Zou Hanfa H  

Scientific reports 20160303


Proteolysis is a major form of post translational modification which occurs when a protease cleaves peptide bonds in a target protein to modify its activity. Tracking protease substrates is indispensable for understanding its cellular functions. However, it is difficult to directly identify protease substrates because the end products of proteolysis, the cleaved protein fragments, must be identified among the pool of cellular proteins. Here we present a bead-based cleavage approach using immobil  ...[more]

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