Ontology highlight
ABSTRACT:
SUBMITTER: Wang C
PROVIDER: S-EPMC4776233 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Wang Chunli C Ye Mingliang M Wei Xiaoluan X Bian Yangyang Y Cheng Kai K Zou Hanfa H
Scientific reports 20160303
Proteolysis is a major form of post translational modification which occurs when a protease cleaves peptide bonds in a target protein to modify its activity. Tracking protease substrates is indispensable for understanding its cellular functions. However, it is difficult to directly identify protease substrates because the end products of proteolysis, the cleaved protein fragments, must be identified among the pool of cellular proteins. Here we present a bead-based cleavage approach using immobil ...[more]