Ontology highlight
ABSTRACT:
SUBMITTER: Jung S
PROVIDER: S-EPMC4780416 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Jung Sunhee S Danziger Samuel A SA Panchaud Alexandre A von Haller Priska P Aitchison John D JD Goodlett David R DR
Journal of proteomics & bioinformatics 20150101 10
Here we used a data-independent acquisition (DIA) method, Precursor Acquisition Independent from Ion Count (PAcIFIC), to systematically profile the <i>S. cerevisiae</i> proteome. Direct PAcIFIC analysis of a yeast whole cell lysate (WCL) yielded 90% reproducibility between replicates and detected approximately 2000 proteins. When combined with sub-cellular fractionation, reproducibility was equally high and the number of detected yeast proteins approached 5000. As noted previously, this unbiased ...[more]