Ontology highlight
ABSTRACT:
SUBMITTER: Miller C
PROVIDER: S-EPMC4782793 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
FEBS letters 20150706 17
Incorporation of selenocysteine (Sec) in bacteria requires a UGA codon that is reassigned to Sec by the Sec-specific elongation factor SelB and a conserved mRNA motif (SECIS element). These requirements severely restrict the engineering of selenoproteins. Earlier, a synthetic tRNASec was reported that allowed canonical Sec incorporation by EF-Tu; however, serine misincorporation limited its scope. We report a superior tRNASec variant (tRNAUTuX) that facilitates EF-Tu dependent stoichiometric Sec ...[more]