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Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.

ABSTRACT: We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50?nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3?g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.

SUBMITTER: Conesa JJ

PROVIDER: S-EPMC4785355 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.

Conesa Jose Javier JJ Otón Joaquín J Chiappi Michele M Carazo Jose María JM Pereiro Eva E Chichón Francisco Javier FJ Carrascosa José L JL

Scientific reports 20160310

We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the d ...[more]

PMID: 26960695

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