Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.
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ABSTRACT:
SUBMITTER: Cheong TC
PROVIDER: S-EPMC4786874 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.
Cheong Taek-Chin TC Compagno Mara M Chiarle Roberto R
Nature communications 20160309
Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes. By delivering Cas9 and guide-RNA (gRNA) with retro- or lenti-virus to IgM(+) mouse B cells and hybridomas, we induce class-switch recombination (CSR) of the IgH chain to the desired subclass. Similarly ...[more]
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