Ontology highlight
ABSTRACT:
SUBMITTER: Lim CT
PROVIDER: S-EPMC4787754 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Nucleic acids research 20150828 20
The Mre11/Rad50 complex is a central player in various genome maintenance pathways. Here, we report a novel mode of nuclease action found for the Escherichia coli Mre11/Rad50 complex, SbcC2/D2 complex (SbcCD). SbcCD cuts off the top of a cruciform DNA by making incisions on both strands and continues cleaving the dsDNA stem at ∼10-bp intervals. Using linear-shaped DNA substrates, we observed that SbcCD cleaved dsDNA using this activity when the substrate was 110 bp long, but that on shorter subs ...[more]