Unknown

Dataset Information

0

Structural basis for selective targeting of leishmanial ribosomes: aminoglycoside derivatives as promising therapeutics.


ABSTRACT: Leishmaniasis comprises an array of diseases caused by pathogenic species of Leishmania, resulting in a spectrum of mild to life-threatening pathologies. Currently available therapies for leishmaniasis include a limited selection of drugs. This coupled with the rather fast emergence of parasite resistance, presents a dire public health concern. Paromomycin (PAR), a broad-spectrum aminoglycoside antibiotic, has been shown in recent years to be highly efficient in treating visceral leishmaniasis (VL)-the life-threatening form of the disease. While much focus has been given to exploration of PAR activities in bacteria, its mechanism of action in Leishmania has received relatively little scrutiny and has yet to be fully deciphered. In the present study we present an X-ray structure of PAR bound to rRNA model mimicking its leishmanial binding target, the ribosomal A-site. We also evaluate PAR inhibitory actions on leishmanial growth and ribosome function, as well as effects on auditory sensory cells, by comparing several structurally related natural and synthetic aminoglycoside derivatives. The results provide insights into the structural elements important for aminoglycoside inhibitory activities and selectivity for leishmanial cytosolic ribosomes, highlighting a novel synthetic derivative, compound 3: , as a prospective therapeutic candidate for the treatment of VL.

SUBMITTER: Shalev M 

PROVIDER: S-EPMC4787808 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structural basis for selective targeting of leishmanial ribosomes: aminoglycoside derivatives as promising therapeutics.

Shalev Moran M   Rozenberg Haim H   Smolkin Boris B   Nasereddin Abedelmajeed A   Kopelyanskiy Dmitry D   Belakhov Valery V   Schrepfer Thomas T   Schacht Jochen J   Jaffe Charles L CL   Adir Noam N   Baasov Timor T  

Nucleic acids research 20150811 17


Leishmaniasis comprises an array of diseases caused by pathogenic species of Leishmania, resulting in a spectrum of mild to life-threatening pathologies. Currently available therapies for leishmaniasis include a limited selection of drugs. This coupled with the rather fast emergence of parasite resistance, presents a dire public health concern. Paromomycin (PAR), a broad-spectrum aminoglycoside antibiotic, has been shown in recent years to be highly efficient in treating visceral leishmaniasis (  ...[more]

Similar Datasets

| S-EPMC7037830 | biostudies-literature
| S-EPMC9424295 | biostudies-literature
| S-EPMC8320071 | biostudies-literature
| S-EPMC8570131 | biostudies-literature
| S-EPMC6345968 | biostudies-literature
| S-EPMC2836080 | biostudies-literature
| S-EPMC4202025 | biostudies-literature
| S-EPMC4313529 | biostudies-literature
| S-EPMC9302021 | biostudies-literature
| S-EPMC2704631 | biostudies-literature