Unknown

Dataset Information

0

A Gain-Of-Function Mutation in the Plcg2 Gene Protects Mice from Helicobacter felis-Induced Gastric MALT Lymphoma.


ABSTRACT: Gastric mucosa-associated lymphoid tissue (MALT) lymphomas develop from a chronic Helicobacter infection. Phospholipase C gamma 2 (PLCG2) is important for B-cell survival and proliferation. We used BALB/c mice with a gain-of-function mutation in the Plcg2 gene (Ali5) to analyze its role in the development of gastric MALT lymphoma. Heterozygous BALB/c Plcg2Ali5/+ and wildtype (WT) mice were infected with Helicobacter felis (H. felis) and observed up to 16 months for development of gastric MALT lymphomas. In contrast to our initial hypothesis, Plcg2Ali5/+ mice developed MALT lymphomas less frequently than their WT littermates after long-term infection of 16 months. Infected Plcg2Ali5/+ mice showed downregulation of proinflammatory cytokines and decreased H. felis-specific IgG1 and IgG2a antibody responses. These results suggested a blunted immune response of Plcg2Ali5/+ mice towards H. felis infection. Intriguingly, Plcg2Ali5/+ mice harboured higher numbers of CD73 expressing regulatory T cells (Tregs), possibly responsible for impaired immune response towards Helicobacter infection. We suggest that Plcg2Ali5/+ mice may be protected from developing gastric MALT lymphomas as a result of elevated Treg numbers, reduced response to H. felis and decrease of proinflammatory cytokines.

SUBMITTER: Gossmann J 

PROVIDER: S-EPMC4788355 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Gain-Of-Function Mutation in the Plcg2 Gene Protects Mice from Helicobacter felis-Induced Gastric MALT Lymphoma.

Gossmann Jennifer J   Stolte Manfred M   Lohoff Michael M   Yu Philipp P   Moll Roland R   Finkernagel Florian F   Garn Holger H   Brendel Cornelia C   Bittner Alwina A   Neubauer Andreas A   Huynh Minh Q MQ  

PloS one 20160311 3


Gastric mucosa-associated lymphoid tissue (MALT) lymphomas develop from a chronic Helicobacter infection. Phospholipase C gamma 2 (PLCG2) is important for B-cell survival and proliferation. We used BALB/c mice with a gain-of-function mutation in the Plcg2 gene (Ali5) to analyze its role in the development of gastric MALT lymphoma. Heterozygous BALB/c Plcg2Ali5/+ and wildtype (WT) mice were infected with Helicobacter felis (H. felis) and observed up to 16 months for development of gastric MALT ly  ...[more]

Similar Datasets

| S-EPMC6616415 | biostudies-literature
| S-EPMC7485896 | biostudies-literature
| S-EPMC3959547 | biostudies-literature
| S-EPMC6998799 | biostudies-literature
| S-EPMC1187288 | biostudies-literature
| S-EPMC4823114 | biostudies-literature
| S-EPMC5101760 | biostudies-literature
| S-EPMC8632687 | biostudies-literature
| S-EPMC5533599 | biostudies-other
2020-04-07 | GSE148112 | GEO