Ontology highlight
ABSTRACT:
SUBMITTER: Chai KP
PROVIDER: S-EPMC4791539 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Chai Kian Piaw KP Othman Noor Farhan Binti NF Teh Aik-Hong AH Ho Kok Lian KL Chan Kok-Gan KG Shamsir Mohd Shahir MS Goh Kian Mau KM Ng Chyan Leong CL
Scientific reports 20160315
A new subfamily of glycosyl hydrolase family GH13 was recently proposed for α-amylases from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo, and GtamyII), Bacillus aquimaris (BaqA), and 95 other putative protein homologues. To understand this new GH13 subfamily, we report crystal structures of truncated ASKA (TASKA). ASKA is a thermostable enzyme capable of producing high levels of maltose. Unlike GTA, biochemical analysis showed that Ca(2+) ion supplementation enh ...[more]