Ontology highlight
ABSTRACT:
SUBMITTER: Ranjani V
PROVIDER: S-EPMC5376179 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Ranjani Velayudhan V Janeček Stefan S Chai Kian Piaw KP Shahir Shafinaz S Abdul Rahman Raja Noor Zaliha Raja RN Chan Kok-Gan KG Goh Kian Mau KM
Scientific reports 20140728
The α-amylases from Anoxybacillus species (ASKA and ADTA), Bacillus aquimaris (BaqA) and Geobacillus thermoleovorans (GTA, Pizzo and GtamyII) were proposed as a novel group of the α-amylase family GH13. An ASKA yielding a high percentage of maltose upon its reaction on starch was chosen as a model to study the residues responsible for the biochemical properties. Four residues from conserved sequence regions (CSRs) were thus selected, and the mutants F113V (CSR-I), Y187F and L189I (CSR-II) and A1 ...[more]