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MARCKS-like protein is an initiating molecule in axolotl appendage regeneration.

ABSTRACT: Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.

SUBMITTER: Sugiura T

PROVIDER: S-EPMC4795554 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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MARCKS-like protein is an initiating molecule in axolotl appendage regeneration.

Sugiura Takuji T Wang Heng H Barsacchi Rico R Simon Andras A Tanaka Elly M EM

Nature 20160301 7593

Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial ...[more]

PMID: 26934225

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