Dataset Information

Comparison of Protein Expression During Early Phases of Limb Regeneration Between Regeneration-permissive and Regeneration-deficient Axolotl

ABSTRACT: In this project, we studied the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by means of LC-MS/MS technology. We quantified a total of 714 proteins having an adjusted p < 0.01 with FC greater or equal to 2 between two conditions. Principal component analysis revealed a conspicuous clustering between neotenic and metamorphic samples at 7 days post-amputation. Different set of proteins was identified as differentially expressed at all of the time points (1, 4, and 7 days post-amputations against day0) for neotenic and metamorphic stages. Although functional enrichment analyses underline the presence of common pathways between regenerative and non-regenerative stages, cell proliferation and its regulation associated pathways, immune system related pathways and muscle tissue and ECM remodeling and degradation pathways were represented at different rate between both stages.

INSTRUMENT(S): SYNAPT G2-Si, ACQUITY UPLC

ORGANISM(S): Ambystoma Mexicanum

TISSUE(S): Limb, Blastema

SUBMITTER: Baris Suzek

LAB HEAD: Turan Demircan

PROVIDER: PXD014806 | Pride | 2019-11-25

REPOSITORIES: Pride

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Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl.

Sibai Mustafa M Altuntaş Ebru E Süzek Barış Ethem BE Şahin Betül B Parlayan Cüneyd C Öztürk Gürkan G Baykal Ahmet Tarık AT Demircan Turan T

Biochemical and biophysical research communications 20191122 2

The axolotl (Ambystoma mexicanum) salamander, a urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by employing LC-MS/MS techn ...[more]

PMID: 31767146

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