Unknown

Dataset Information

0

A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster.


ABSTRACT: The incorporation of CENP-A into centromeric chromatin is an essential prerequisite for kinetochore formation. Yet, the molecular mechanisms governing this process are surprisingly divergent in different organisms. While CENP-A loading mechanisms have been studied in some detail in mammals, there are still large gaps to our understanding of CENP-A/Cid loading pathways in Drosophila. Here, we report on the characterization and delineation of at least three different CENP-A preloading complexes in Drosophila. Two complexes contain the CENP-A chaperones CAL1, FACT and/or Caf1/Rbap48. Notably, we identified a novel complex consisting of the histone acetyltransferase Hat1, Caf1 and CENP-A/H4. We show that Hat1 is required for proper CENP-A loading into chromatin, since knock-down in S2 cells leads to reduced incorporation of newly synthesized CENP-A. In addition, we demonstrate that CENP-A/Cid interacts with the HAT1 complex via an N-terminal region, which is acetylated in cytoplasmic but not in nuclear CENP-A. Since Hat1 is not responsible for acetylation of CENP-A/Cid, these results suggest a histone acetyltransferase activity-independent escort function for Hat1. Thus, our results point toward intriguing analogies between the complex processing pathways of newly synthesized CENP-A and canonical histones.

SUBMITTER: Boltengagen M 

PROVIDER: S-EPMC4797270 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4608838 | biostudies-literature
| S-EPMC6718594 | biostudies-literature
| S-EPMC3299411 | biostudies-literature
| S-EPMC6884459 | biostudies-literature
2015-09-25 | GSE73409 | GEO
| S-EPMC5433716 | biostudies-literature
| S-EPMC1461554 | biostudies-other
| S-EPMC3912524 | biostudies-literature
| S-EPMC3531477 | biostudies-literature
| S-EPMC6834884 | biostudies-literature