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SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in A?PP/PS-1 transgenic mouse brain.

ABSTRACT: In our program to develop non-invasive magnetic resonance imaging (MRI) methods for the diagnosis of Alzheimer's disease (AD), we have synthesized antibody-conjugated, superparamagnetic iron oxide nanoparticles (SPIONs) for use as an in vivo agent for MRI detection of amyloid-? plaques in AD. Here we report studies in A?PP/PS1 transgenic mice, which demonstrate the ability of novel anti-A?PP conjugated SPIONs to penetrate the blood-brain barrier to act as a contrast agent for MR imaging of plaques. The conspicuity of the plaques increased from an average Z-score of 5.1 ± 0.5 to 8.3 ± 0.2 when the plaque contrast to noise ratio was compared in control AD mice with AD mice treated with SPIONs. The number of MRI-visible plaques per brain increased from 347 ± 45 in the control AD mice, to 668 ± 86 in the SPION treated mice. These results indicated that our SPION enhanced amyloid-? detection method delivers an efficacious, non-invasive MRI detection method in transgenic mice.

SUBMITTER: Sillerud LO

PROVIDER: S-EPMC4801216 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in AβPP/PS-1 transgenic mouse brain.

Sillerud Laurel O LO Solberg Nathan O NO Chamberlain Ryan R Orlando Robert A RA Heidrich John E JE Brown David C DC Brady Christina I CI Vander Jagt Thomas A TA Garwood Michael M Vander Jagt David L DL

Journal of Alzheimer's disease : JAD 20130101 2

In our program to develop non-invasive magnetic resonance imaging (MRI) methods for the diagnosis of Alzheimer's disease (AD), we have synthesized antibody-conjugated, superparamagnetic iron oxide nanoparticles (SPIONs) for use as an in vivo agent for MRI detection of amyloid-β plaques in AD. Here we report studies in AβPP/PS1 transgenic mice, which demonstrate the ability of novel anti-AβPP conjugated SPIONs to penetrate the blood-brain barrier to act as a contrast agent for MR imaging of plaqu ...[more]

PMID: 23229079

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Dataset Information

SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in A?PP/PS-1 transgenic mouse brain.

Publications

SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in AβPP/PS-1 transgenic mouse brain.

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