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Morphologic effects of estrogen stimulation on 3D MCF-7 microtissues.


ABSTRACT: In the development of human cell-based assays, 3-dimensional (3D) cell culture models are intriguing as they are able to bridge the gap between animal models and traditional two-dimensional (2D) cell culture. Previous work has demonstrated that MCF-7 human breast carcinoma cells cultured in a 3D scaffold-free culture system self-assemble and develop into differentiated microtissues that possess a luminal space. Exposure to estradiol for 7 days decreased lumen formation in MCF-7 microtissues, altered microtissue morphology and altered expression of genes involved in estrogen signaling, cell adhesion and cell cycle regulation. Exposure to receptor-specific agonists for estrogen receptor alpha, estrogen receptor beta and g-protein coupled estrogen receptor resulted in unique, receptor-specific phenotypes and gene expression signatures. The use of a differentiated scaffold-free 3D culture system offers a unique opportunity to study the phenotypic and molecular changes associated with exposure to estrogenic compounds.

SUBMITTER: Vantangoli MM 

PROVIDER: S-EPMC4803074 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Morphologic effects of estrogen stimulation on 3D MCF-7 microtissues.

Vantangoli Marguerite M MM   Wilson Shelby S   Madnick Samantha J SJ   Huse Susan M SM   Boekelheide Kim K  

Toxicology letters 20160224


In the development of human cell-based assays, 3-dimensional (3D) cell culture models are intriguing as they are able to bridge the gap between animal models and traditional two-dimensional (2D) cell culture. Previous work has demonstrated that MCF-7 human breast carcinoma cells cultured in a 3D scaffold-free culture system self-assemble and develop into differentiated microtissues that possess a luminal space. Exposure to estradiol for 7 days decreased lumen formation in MCF-7 microtissues, alt  ...[more]

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