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A strategy for dissecting the architectures of native macromolecular assemblies.

ABSTRACT: It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

SUBMITTER: Shi Y

PROVIDER: S-EPMC4803312 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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A strategy for dissecting the architectures of native macromolecular assemblies.

Shi Yi Y Pellarin Riccardo R Fridy Peter C PC Fernandez-Martinez Javier J Thompson Mary K MK Li Yinyin Y Wang Qing Jun QJ Sali Andrej A Rout Michael P MP Chait Brian T BT

Nature methods 20151005 12

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures. ...[more]

PMID: 26436480

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