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Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

ABSTRACT: Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.

SUBMITTER: Mlodzianoski MJ

PROVIDER: S-EPMC4803349 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

Mlodzianoski Michael J MJ Curthoys Nikki M NM Gunewardene Mudalige S MS Carter Sean S Hess Samuel T ST

PloS one 20160322 3

Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different spe ...[more]

PMID: 27002724

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