Color-Coded Super-Resolution Small-Molecule Imaging.

ABSTRACT: Although the development of super-resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super-resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules.

SUBMITTER: Beuzer P 

PROVIDER: S-EPMC5291120 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature