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Donor substrate promiscuity of bacterial ?1-3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of ?1-4-galactosyltransferases.


ABSTRACT: ?1-3-N-Acetylglucosaminyltransferases (?3GlcNAcTs) and ?1-4-galactosyltransferases (?4GalTs) have been broadly used in enzymatic synthesis of N-acetyllactosamine (LacNAc)-containing oligosaccharides and glycoconjugates including poly-LacNAc, and lacto-N-neotetraose (LNnT) found in the milk of human and other mammals. In order to explore oligosaccharides and derivatives that can be synthesized by the combination of ?3GlcNAcTs and ?4GalTs, donor substrate specificity studies of two bacterial ?3GlcNAcTs from Helicobacter pylori (Hp?3GlcNAcT) and Neisseria meningitidis (NmLgtA), respectively, using a library of 39 sugar nucleotides were carried out. The two ?3GlcNAcTs have complementary donor substrate promiscuity and 13 different trisaccharides were produced. They were used to investigate the acceptor substrate specificities of three ?4GalTs from Neisseria meningitidis (NmLgtB), Helicobacter pylori (Hp?4GalT), and bovine (B?4GalT), respectively. Ten of the 13 trisaccharides were shown to be tolerable acceptors for at least one of these ?4GalTs. The application of NmLgtA in one-pot multienzyme (OPME) synthesis of two trisaccharides including GalNAc?1-3Gal?1-4Glc?ProN3 and Gal?1-3Gal?1-4Glc was demonstrated. The study provides important information for using these glycosyltransferases as powerful catalysts in enzymatic and chemoenzymatic syntheses of oligosaccharides and derivatives which can be useful probes and reagents.

SUBMITTER: Li Y 

PROVIDER: S-EPMC4808627 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Donor substrate promiscuity of bacterial β1-3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1-4-galactosyltransferases.

Li Yanhong Y   Xue Mengyang M   Sheng Xue X   Yu Hai H   Zeng Jie J   Thon Vireak V   Chen Yi Y   Muthana Musleh M MM   Wang Peng G PG   Chen Xi X  

Bioorganic & medicinal chemistry 20160303 8


β1-3-N-Acetylglucosaminyltransferases (β3GlcNAcTs) and β1-4-galactosyltransferases (β4GalTs) have been broadly used in enzymatic synthesis of N-acetyllactosamine (LacNAc)-containing oligosaccharides and glycoconjugates including poly-LacNAc, and lacto-N-neotetraose (LNnT) found in the milk of human and other mammals. In order to explore oligosaccharides and derivatives that can be synthesized by the combination of β3GlcNAcTs and β4GalTs, donor substrate specificity studies of two bacterial β3Glc  ...[more]

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