The Roles of Histidines and Charged Residues as Potential Triggers of a Conformational Change in the Fusion Loop of Ebola Virus Glycoprotein.
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ABSTRACT:
SUBMITTER: Lee J
PROVIDER: S-EPMC4811418 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
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The Roles of Histidines and Charged Residues as Potential Triggers of a Conformational Change in the Fusion Loop of Ebola Virus Glycoprotein.
Lee Jinwoo J Gregory Sonia M SM Nelson Elizabeth A EA White Judith M JM Tamm Lukas K LK
PloS one 20160329 3
Ebola virus (EBOV) enters cells from late endosomes/lysosomes under mildly acidic conditions. Entry by fusion with the endosomal membrane requires the fusion loop (FL, residues 507-560) of the EBOV surface glycoprotein to undergo a pH-dependent conformational change. To find the pH trigger for this reaction we mutated multiple conserved histidines and charged and uncharged hydrophilic residues in the FL and measured their activity by liposome fusion and cell entry of virus-like particles. The FL ...[more]
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