Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers.
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ABSTRACT:
SUBMITTER: Labouesse C
PROVIDER: S-EPMC4812326 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers.
Labouesse Céline C Gabella Chiara C Meister Jean-Jacques JJ Vianay Benoît B Verkhovsky Alexander B AB
Scientific reports 20160330
Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous ...[more]
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