Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC4813330 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Cell 20151101 5
Nearly half of the ribosomes translating a particular bacteriophage T4 mRNA bypass a region of 50 nt, resuming translation 3' of this gap. How this large-scale, specific hop occurs and what determines whether a ribosome bypasses remain unclear. We apply single-molecule fluorescence with zero-mode waveguides to track individual Escherichia coli ribosomes during translation of T4's gene 60 mRNA. Ribosomes that bypass are characterized by a 10- to 20-fold longer pause in a non-canonical rotated sta ...[more]