Ontology highlight
ABSTRACT:
SUBMITTER: Li M
PROVIDER: S-EPMC4815411 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20160105 3
Determining the energetics of the unfolded state of a protein is essential for understanding the folding mechanics of ordered proteins and the structure-function relation of intrinsically disordered proteins. Here, we adopt a coil-globule transition theory to develop a general scheme to extract interaction and free energy information from single-molecule fluorescence resonance energy transfer spectroscopy. By combining protein stability data, we have determined the free energy difference between ...[more]