Hypermethylation of genes in testicular embryonal carcinomas.

ABSTRACT:

Background

Testicular embryonal carcinoma (EC) is a major subtype of non-seminomatous germ cell tumours in males. Embryonal carcinomas are pluripotent, undifferentiated germ cell tumours believed to originate from primordial germ cells. Epigenetic changes during testicular EC tumorigenesis require better elucidation.

Methods

To identify epigenetic changes during testicular neoplastic transformation, we profiled DNA methylation of six ECs. These samples represent different stages (stage I and stage III) of divergent invasiveness. Non-cancerous testicular tissues were included. Expression of a number of hypermethylated genes were examined by quantitative RT-PCR and immunohistochemistry (IHC).

Results

A total of 1167 tumour-hypermethylated differentially methylated regio

SUBMITTER: Cheung HH 

PROVIDER: S-EPMC4815804 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature