Ontology highlight
ABSTRACT:
SUBMITTER: Zhu G
PROVIDER: S-EPMC4816075 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Zhu Guijie G Sun Liangliang L Heidbrink-Thompson Jennifer J Kuntumalla Srilatha S Lin Hung-yu HY Larkin Christopher J CJ McGivney James B JB Dovichi Norman J NJ
Electrophoresis 20160201 4
We have evaluated CZE-ESI-MS/MS for detection of trace amounts of host cell protein impurities in recombinant therapeutics. Compared to previously published procedures, we have optimized the buffer pH used in the formation of a pH junction to increase injection volume. We also prepared a 5-point calibration curve by spiking 12 standard proteins into a solution of a human mAb. A custom CZE-MS/MS system was used to analyze the tryptic digest of this mixture without depletion of the antibody. CZE g ...[more]