Dataset Information

Complete Genome Sequence of a ?-Hexachlorocyclohexane Degrader, Sphingobium sp. Strain TKS, Isolated from a ?-Hexachlorocyclohexane-Degrading Microbial Community.

ABSTRACT: Here, we report the complete genome sequence of a ?-hexachlorocyclohexane (?-HCH) degrader,Sphingobiumsp. strain TKS, which was isolated from a ?-HCH-degrading microbial community. The genome of TKS consists of two chromosomes and nine plasmids. Thelingenes for conversion of ?-HCH to ?-ketoadipate are dispersed on chromosome 1 and three out of the nine plasmids.

SUBMITTER: Tabata M

PROVIDER: S-EPMC4824264 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Publications

Complete Genome Sequence of a γ-Hexachlorocyclohexane Degrader, Sphingobium sp. Strain TKS, Isolated from a γ-Hexachlorocyclohexane-Degrading Microbial Community.

Tabata Michro M Ohhata Satoshi S Kawasumi Toru T Nikawadori Yuki Y Kishida Kouhei K Sato Takuya T Ohtsubo Yoshiyuki Y Tsuda Masataka M Nagata Yuji Y

Genome announcements 20160407 2

Here, we report the complete genome sequence of a γ-hexachlorocyclohexane (γ-HCH) degrader,Sphingobiumsp. strain TKS, which was isolated from a γ-HCH-degrading microbial community. The genome of TKS consists of two chromosomes and nine plasmids. Thelingenes for conversion of γ-HCH to β-ketoadipate are dispersed on chromosome 1 and three out of the nine plasmids. ...[more]

PMID: 27056231

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Project description:Among sphingomonads, Sphingobium indicum B90A is widely investigated for its ability to degrade a manmade pesticide, γ-hexachlorocyclohexane (γ-HCH) and its isomers (α-, β-, δ-, and ε-HCH). In this study, complete genome of strain B90A was constructed using Single Molecule Real Time Sequencing (SMRT) and Illumina platform. The complete genome revealed that strain B90A harbors four replicons: one chromosome (3,654,322 bp) and three plasmids designated as pSRL1 (139,218 bp), pSRL2 (108,430 bp) and pSRL3 (43,761 bp). The study determined the precise location of lin genes (genes associated with the degradation of HCH isomers), for example, linA2, linB, linDER, linF, linGHIJ, and linKLMN on the chromosome; linA1, linC, and linF on pSRL1 and linDEbR on pSRL3. Strain B90A contained 26 copies of IS6100 element and most of them (15 copies) was found to be associated with lin genes. Duplication of several lin genes including linA, linDER, linGHIJ, and linF along with two variants of linE, that is, linEa (hydroquinone 1,2-dioxygenase) and linEb (chlorohydroquinone/hydroquinone 1,2-dioxygenase) were identified. This suggests that strain B90A not only possess efficient machinery for upper and lower HCH degradation pathways but it can also act on both hydroquinone and chlorohydroquinone metabolites produced during γ-HCH degradation. Synteny analysis revealed the duplication and transposition of linA gene (HCH dehydrochlorinase) between the chromosome and pSRL1, possibly through homologous recombination between adjacent IS6100 elements. Further, in silico analysis and laboratory experiments revealed that incomplete tyrosine metabolism was responsible for the production of extracellular brown pigment which distinguished strain B90A from other HCH degrading sphingomonads. The precise localization of lin genes, and transposable elements (IS6100) on different replicons now opens up several experimental avenues to elucidate the functions and regulatory mechanism of lin genes acquisition and transfer that were not completely known among the bacterial population inhabiting the HCH contaminated environment.

Dataset Information

Complete Genome Sequence of a ?-Hexachlorocyclohexane Degrader, Sphingobium sp. Strain TKS, Isolated from a ?-Hexachlorocyclohexane-Degrading Microbial Community.

Publications

Complete Genome Sequence of a γ-Hexachlorocyclohexane Degrader, Sphingobium sp. Strain TKS, Isolated from a γ-Hexachlorocyclohexane-Degrading Microbial Community.

Similar Datasets

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