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Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.

ABSTRACT: RNA nanotechnology uses RNA structural motifs to build nanosized architectures that assemble through selective base-pair interactions. Herein, we report the crystal-structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6?Å resolution reveals the so-far smallest circularly closed nanoobject made entirely of double-stranded RNA. The assembly of the nanotriangle architecture involved RNA corner motifs that were derived from ligand-responsive RNA switches, which offer the opportunity to control self-assembly and dissociation.

SUBMITTER: Boerneke MA

PROVIDER: S-EPMC4824544 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.

Boerneke Mark A MA Dibrov Sergey M SM Hermann Thomas T

Angewandte Chemie (International ed. in English) 20160223 12

RNA nanotechnology uses RNA structural motifs to build nanosized architectures that assemble through selective base-pair interactions. Herein, we report the crystal-structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6 Å resolution reveals the so-far smallest circularly closed nanoobject made entirely of double-stranded RNA. The assembly of the nanotriangle ...[more]

PMID: 26914842

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