Ontology highlight
ABSTRACT:
SUBMITTER: Nelles DA
PROVIDER: S-EPMC4826288 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Nelles David A DA Fang Mark Y MY O'Connell Mitchell R MR Xu Jia L JL Markmiller Sebastian J SJ Doudna Jennifer A JA Yeo Gene W GW
Cell 20160317 2
RNA-programmed genome editing using CRISPR/Cas9 from Streptococcus pyogenes has enabled rapid and accessible alteration of specific genomic loci in many organisms. A flexible means to target RNA would allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic tools, but most RNA targeting methods rely on incorporation of exogenous tags. Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and al ...[more]