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DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.

ABSTRACT: Progress in whole-genome sequencing using short-read (e.g., <150?bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new "nanomapping" method to detect and map precisely BCL2-IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM "nanomapping" technique can be complementary to both sequencing and other physical mapping approaches.

SUBMITTER: Mikheikin A

PROVIDER: S-EPMC5698298 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.

Mikheikin Andrey A Olsen Anita A Leslie Kevin K Russell-Pavier Freddie F Yacoot Andrew A Picco Loren L Payton Oliver O Toor Amir A Chesney Alden A Gimzewski James K JK Mishra Bud B Reed Jason J

Nature communications 20171121 1

Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD ...[more]

PMID: 29162844

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